An optimized approach to germ-free rearing in the jewel wasp Nasonia
Development of a Nasonia Kids Balboa HOODIES in vitrogerm-free rearing system in 2012 enabled investigation of Nasonia-microbiota interactions and real-time visualization of parasitoid metamorphosis.However, the use of antibiotics, bleach, and fetal bovine serum introduced artifacts relative to conventional rearing of Nasonia.Here, we optimize the